Effect of Heavy Metals on the Activity of Catalase and Glutathione-S-Transferase in Nile Tilapia Fish (Oreochromis niloticus)

Le Thu Ha, Bui Thi Hoa, Pham Thi Dau

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Published Dec 7, 2021
DOI: https://doi.org/10.25073/2588-1140/vnunst.5339

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How to Cite
HA, Le Thu; HOA, Bui Thi; DAU, Pham Thi. Effect of Heavy Metals on the Activity of Catalase and Glutathione-S-Transferase in Nile Tilapia Fish (Oreochromis niloticus). VNU Journal of Science: Natural Sciences and Technology, [S.l.], v. 37, n. 4, dec. 2021. ISSN 2588-1140. Available at: <https://js.vnu.edu.vn/NST/article/view/5339>. Date accessed: 26 dec. 2024. doi: https://doi.org/10.25073/2588-1140/vnunst.5339.
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Issue
Vol 37 No 4
Section
Original Articles

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Abstract

This study evaluated the response of antioxidant enzymes, such as Catalase (CAT) and Glutathione-S-transferase (GST) in freshwater Nile tilapia fish Oreochromis niloticus (O. niloticus) exposed to heavy metals (HMs) including copper (Cu), lead (Pb) and cadmium (Cd). Fish were expose to various concentrations of Cu2+, Pb2+ (0, 0.02, 0.05, 0.2 mg/l) and Cd2+ (0, 0.005, 0.01, 0.05 mg/l) for 15, 30, 45 and 60 days. The results indicated that enzyme activity was different according to the exposure time, concentration and type of heavy metals. CAT activity increased significantly beginning at day 45 of HMs exposure. After 60 days of exposure, CAT activity was steady with Cu and Pb but inhibited by Cd. While, GST induction was earlier observed from day 15 of HMs exposure. The increase of GST activity was found with the increase of exposure time in the treatment with Cu and Cd but not with Pb. Interestingly, GST activity was inhibited by Pb at longer exposure (45 and 60 days). Among tested metals, Cu showed the weaker effect on the activity of CAT and GST in comparison with Pb and Cd suggesting that these enzymes were less sensitive to Cu than other tested metals.

Keywords: Heavy metal, Catalase, Glutathione-S-Transferase, Oreochromis niloticus.

References

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