Structural Characteristics and Antioxidant Activities of Polysaccharide Extracted from Ulva fasciata Green Seaweed
Main Article Content
Abstract
In this study, the structural characteristics of a polysaccharide extracted from the Ulva fasciata green seaweed were determined using the chemical composition analytical methods combined with IR, MS, and NMR spectra. The results showed that the polysaccharide consists of two major repeating disaccharide units designated as ulvanobiuronic acid 3-sulfate type A (A3s) [→4)-β-D-GlcA-(1 → 4)-α-L-Rha 3S-(1→] and type B (B3s) [→4)-α-LIdoA-(1 → 4)-α-L-Rha 3s(1→]. This kind of polysaccharide exhibited a significant in vitro antioxidant activity. This study demonstrated that the Ulva fasciata green algae can be considered as a source of bioactive ulvan.
Keywords:
Polysaccharide, structure, antioxidant activity, green algae.
References
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[25] A. D. Robic, J. F Bertrand, Y. Sassi, M. Lerat, J. Lahay, Determination of the Chemical Composition of Ulvan, a Cell Wall Polysaccharide from Ulva spp. (Ulvales, Chlorophyta) by FT-IR and Chemometrics, J. appl. Phycol., Vol. 21, 2009, 451-456.
[26] T. Mehdi, L. S. Joon, S. G. You, Structural Analysis of Immunostimulating Sulfated Polysaccharides from Ulva pertusa, Carbohydrate Research, Vol. 361, 2012, pp. 141-147.
[27] D. Revathi, K. Baskaran, R. Subashini, Antioxidant and Free Radical Scavenging Capacity of Red Seaweed Hypnea valentiae from Rameshwaram Coast, Tamil Nadu, India, International Journal of Pharmacy and Pharmaceutical Sciences, 2015, pp. 232-237.
[28] S. K. Chandini, P. Ganesan, N. Bhaskar, In Vitro Antioxidant Activities of Three Selected Brown Seaweeds of India, Food Chem., Vol. 107, 2008, pp. 707-713.
[29] P. Shao, Y. Pei, Z. Fang, P. Sun, Effects of partial desulfation on antioxidant and inhibition of DLD cancer cell of Ulva fasciata polysaccharide, Int. J. Biol, Macromol., Vol. 65, 2014, pp. 307-313.
[2] L. Mata, M. Magnusson, N. A. Paul, R. de Nys, The Intensive Land-based Production of the Green Seaweeds Derbesia tenuissima and Ulva ohnoi: Biomass and Bioproducts, Journal of Applied Phycology, Vol. 28, 2016, pp. 365-375.
[3] J. Blomster, S. Bäck, D. P. Fewer, M. Kiirikki, A. Lehvo, C. A. Maggs, M. J. Stanhope, Novel Morphology in Enteromorpha (Ulvophyceae) Forming Green Tides, Am. J. Bot., Vol. 89, 2002, pp. 1756-1763.
[4] A. E. Nardelli, V. G. Chiozzini, E. S. Braga, F. Chow, Integrated Multi-trophic Farming System Between the Green Seaweed Ulva Lactuca, Mussel, and Fish: A Production and Bioremediation Solution, Journal of Applied Phycology, Vol. 31, 2019, pp. 847-856.
[5] L. Cunha, A. Grenha, Sulfated Seaweed Polysaccharides as Multifunctional Materials in Drug Delivery Applications, Marine Drugs, Vol. 14, No. 3, 2016, pp. 42.
[6] I. Wijesekara, R. Pangestuti, S. K. Kim, Biological Activities and Potential Health Benefits of Sulfated Polysaccharides Derived from Marine Algae, Carbohydrate Polymer, Vol. 84, No. 1, 2011, pp. 14-21.
[7] Y. Chi, H. Li, P. Wang, C. Du, H. Ye, S. Zuo, H. Guan, P. Wang, Structural Characterization of Ulvan Extracted from Ulva clathrata Assisted by an Ulvan Lyase, Carbohydrate Polymers, Vol. 229, 2020, pp. 115497.
[8] C. Costa, A. Alves, P. R. Pinto, R. A. Sousa, E. A. B. da Silva, R. L. Reis, A. E. Rodrigues, Characterization of Ulvan Extracts to Assess the Effect of Different Steps in the Extraction Procedure, Carbohydrate Polymers, Vol. 88, 2012, pp. 537-546.
[9] M. Lahaye, A. Robic, Structure and Function Properties of Ulvan, A Polysaccharide from Green Seaweeds, Biomacromolecules, Vol. 8, 2007, pp. 1765-1774.
[10] R. A. Rosas, L. E. A. Rosas, F. F. Pedroche, Ulva Fasciata Delile (Ulvaceae, Chlorophycota): A Species Newly Introduced Into Pacific Mexico, Botanica Marina, Vol. 48, No. 1, 2005, pp. 46-51.
[11] J. T. Kidgell, M. Magnusson, R. de Nys, C. R. Glasson, Ulvan: A Systematic Review of Extraction, Composition and Function, Algal Research, Vol. 39, 2019, pp. 101422.
[12] K. S. Beach, C. M. Smih, T. Michael, H. W. Shin, Photosythesis in Reproductive Unicells of Ulva fasciata and Enteromorpha flexuosa: Implications for Ecological Success. Mar. Ecol. Prog. Series, Vol. 125, 1995, pp. 229-237.
[13] T. A. Figueira, A. J. R.da Silva, A. E. Prast, Y. Y. Valentin, V. P. de Oliveira, Structural Characterization of Ulvan Polysaccharide from Cultivated and Collected Ulva fasciata (Chlorophyta), Advances in Bioscience and Biotechnology, Vol. 11, No. 5, 2020, pp. 206-216.
[14] Q. N. Huynh, H. D. Nguyen, The Seaweed Resources of Vietnam, In: A. T. Critchley, M. Ohno, Seaweed Resources of the World, JICA, Japan, 1998, pp. 62-69.
[15] D. D. Tien, Study on Seaweed System of Truong Sa Island, PhD Thesis in Biology, Hanoi, 2002 (in Vietnamese).
[16] T. T. T. T. Thanh, Q. T. M. Thi, Thi Nu Nguyen, D. V. Luong, M. L. Bui, T. T. T. Van, Structure and Cytotoxic Activity of Ulvan Extracted from Green Seaweed Ulva lactuca, International Journal of Biological Macromolecules, Vol. 93, 2016, pp. 695-702.
[17] T. T. T. Van, T. B. Hai, N. H. V. Tran, Q. T. T. Minh, N. T. Nu, B. M. Ly, Y. Yuguchi, T. T. T. Thanh, Structure, Conformation in Aqueous Solution and Antimicrobial Activity of Ulvan Extracted from Green Seaweed Ulva reticulata, Natural Product Research, Vol. 32, No. 19, 2018, pp. 2291-2296.
[18] R. P. Reis, A. A. J. Carvalho, A. P. Facchinei, A. C. S. Calheiros, B. Castelar, Direct Effects of Ulvan and a Flour Produced from the Green Alga Ulva Fasciata Delile on the Fungus Stemphylium solani Weber, Algal Research, Vol. 30, 2018, pp. 23-27.
[19] M. I. Bilan, A. A. Grachev, N. E. Ustuzhanina, A Highly Regular Fraction of a Fucoidan from the Brown Seaweed Fucus distichus L., Carbonhydr. Res., Vol. 339, 2004, pp. 511-517.
[20] K. S. Dodgson, Determination of Inorganic Sulphate in Studies on the Enzymic and Non-enzymic Hydrolysis of Carbohydrate and Other Sulphate Esters, Biochem. J., Vol. 78, 1961, pp. 312-319.
[21] T. Bitter, H. M. Muir, A Modified Uronic Acid Carbazole Reaction, Anal, Biochem., Vol. 4, 1962, pp. 330-334.
[22] Q. Y. Zhu, R. M. Hackman, J. L. Ensunsa, R. R. Holt, C. L. Keen, Antioxidative Activities of Olong Tea, J. Agric. Food Chem, Vol. 50, 2002, pp. 6929-6934.
[23] P. Prieto, M. Pineda, M. Aguilar, Spectrophotometric Quantitation of Antioxidant Capacity through the Formation of a Phosphomolybdenum Complex: Specific Application to the Determination of Vitamin E, Anal, Biochem, Vol. 269, 1999, pp. 337-341.
[24] M. S. Blois, Antioxidant Determinations by the use of a Stable Free Radical, Nature, Vol. 181, 1958, pp. 1199-1200.
[25] A. D. Robic, J. F Bertrand, Y. Sassi, M. Lerat, J. Lahay, Determination of the Chemical Composition of Ulvan, a Cell Wall Polysaccharide from Ulva spp. (Ulvales, Chlorophyta) by FT-IR and Chemometrics, J. appl. Phycol., Vol. 21, 2009, 451-456.
[26] T. Mehdi, L. S. Joon, S. G. You, Structural Analysis of Immunostimulating Sulfated Polysaccharides from Ulva pertusa, Carbohydrate Research, Vol. 361, 2012, pp. 141-147.
[27] D. Revathi, K. Baskaran, R. Subashini, Antioxidant and Free Radical Scavenging Capacity of Red Seaweed Hypnea valentiae from Rameshwaram Coast, Tamil Nadu, India, International Journal of Pharmacy and Pharmaceutical Sciences, 2015, pp. 232-237.
[28] S. K. Chandini, P. Ganesan, N. Bhaskar, In Vitro Antioxidant Activities of Three Selected Brown Seaweeds of India, Food Chem., Vol. 107, 2008, pp. 707-713.
[29] P. Shao, Y. Pei, Z. Fang, P. Sun, Effects of partial desulfation on antioxidant and inhibition of DLD cancer cell of Ulva fasciata polysaccharide, Int. J. Biol, Macromol., Vol. 65, 2014, pp. 307-313.