Structural Characteristics and Cytotoxic activity of Polysaccharide Extracted from Green Seaweed Caulerpa torta
Main Article Content
Abstract
In this work, hot water extraction was applied to isolate a polysaccharide from green seaweed Caulerpa torta, the extraction yield was 3.4% of dried seaweed weight. The structural characteristics and cytotoxic effects on three human cancer cell lines of the polysaccharide were investigated. The results showed that the polysaccharide consisted of galactose, glucose, xylose and mannose in a molar ratio of 1.00: 0.74: 0.21: 0.0.21, with a molecular weight of 107 kDa. In addition, it contained sulfate groups, which is approximately 15.34%w. The polysaccharide showed a significant cytotoxic activity against thyroid cancer - 8505C (IC50 55.55±1.84 µg/ml), colon cancer - HT-29 (IC50 57.26±2.06 µg/ml) and cervical cancer - Hela (IC50 54.79 ±3.22 µg/ml).
Keywords:
Polysaccharide, green seaweed, Caulerpa torta, structural characteristics, cytotoxic activity.
References
[1] Trần Đình Toại, Châu Văn Minh, Tiềm năng rong biển Việt Nam, Nhà xuất bản Khoa học và Kỹ thuật, 2004.
[2] Dang Diem Hong, Hoang Minh Hien, Pham Ngoc Son, Use of Vietnamese seaweed for functional food, medicine and biofertilizer, Journal Applied Phycology, 2007, Vol. 19, No. 6, pp. 817-826.
[3] Lingchong Wang, Xiangyu Wang, Hao Wu and Rui Liu, Overview on Biological Activities and Molecular Characteristics of Sulfated Polysaccharides from Marine Green Algae in Recent Years, Mar. Drugs, 2014, Vol. 12, pp. 4984-5020.
[4] Hua Tian, Haifang Liu, Weikang Song, Li Zhu & Xueqiong Yin, Polysaccharide from Caulerpa lentillifera: extraction optimization with response surface methodology, structure and antioxidant activities, Natural Product Research, 2019, DOI:10.1080/14786419.2019.1700507
[5] Costa, M.S.S.P.; Almeida-Lima, J.; Farias, E.H.C., Biological activities of sulfated polysaccharides from tropical seaweeds, Biomed. Pharmacother., 2010, Vol. 64, pp. 21–28.
[6] Kausik Chattopadhyay, Utpal Adhikari, Patrice Lerouge, Bimalendu Ray, Polysaccharides from Caulerpa racemosa: Purification and structural features, Carbohydrate Polymers, 2007, Vol. 68, No. 3, pp. 407-415.
[7] Quach Thi Minh Thu, Truong Hai Bang, Nguyen Thi Nu, Đang Vu Luong, Bui Minh Ly, Tran Thi Thanh Van and Thanh Thi Thu Thuy, Structural Determination of Ulvan from Green Seaweed Ulva reticulata Collected at Central Coast of Vietnam, Chemistry Letters, 2015, Vol. 44, No. 6, pp. 788-790.
[8] Thi Thu Thuy Thanh, Thi Minh Thu Quach, Thi Nu Nguyen, Dang Vu Luong, Bui Minh Ly and Thi Thanh Van Tran, Structure and cytotoxic activity of ulvan extracted from green seaweed Ulva lactuca, International Journal of Biological Macromolecules, 2016, Vol. 93, pp. 695-703.
[9] Quách Thị Minh Thu, Nguyễn Hải Minh, Đỗ Thị Thanh Xuân, Đặng Vũ Lương, Trần Thị Thanh Vân, Nguyễn Thị Nụ, Thành Thị Thu Thủy, Sulfate polysaccharide chiết tách từ rong lục chi Cheatomorpha: đặc điểm cấu trúc và hoạt tính chống đông tụ máu, Tạp chí Hóa học, 2019, Vol. 57, No 6E1,2, pp. 117-120.
[10] Quách Thị Minh Thu, Đặng Vũ Lương, Đỗ Thị Thanh Xuân, Nguyễn Quang Tâm, Ngô Văn Quang, Trần Thị Thanh Vân và Thành Thị Thu Thủy, Đặc trưng cấu trúc của polysaccharide chiết tách từ rong lục Enteromorpha intestinalis, Tạp chí Hóa học, 2019, Vol. 57, No 6E1, 2, pp. 113-116.
[11] A. M. Staub, “Removeal of Protein-Sevag Method,” Methods in Carbohydrate Chemistry, 1965, Vol. 5, pp. 5-6.
[12] Bilan MI., Grachev AA, Ustuzhanina NE., A highly regular fraction of a fucoidan from the brown seaweed Fucus distichus L, Carbonhydr Res., 2004, Vol. 339, pp. 511-517.
[13] Dodgson KS, Determination of inorganic sulphate in studies on the enzymic and non-enzymic hydrolysis of carbohydrate and other sulphate esters, Biochem J., 1961, Vol. 78, pp. 312-319.
[14] Philip Skehan, Ritsa Storeng, Dominic Scudiero, Anne Monks, James McMahon, David Vistica, Jonathan T. Warren, Heidi Bokesch, Susan Kenney, Michael R. Boyd, New Colorimetric Cytotoxicity Assay for Anticancer-Drug Screening, Journal of the National Cancer Institute, 1990, Vol. 82, No. 13, pp. 1107-1112.
[15] José Ariévilo Gurgel Rodrigues, Edfranck de Sousa Oliveira Vanderlei, Érika Façanha
Bessa, Francisco de Araújo Magalhães, Regina Célia Monteiro de Paula, Vilma Lima
and Norma Maria Barros Benevides, Anticoagulant Activity of a Sulfated Polysaccharide Isolated from the Green Seaweed Caulerpa cupressoides, Brazilian Archives of Biology and Technology, 2011, Vol. 54, No. 4, pp. 691-700.
[2] Dang Diem Hong, Hoang Minh Hien, Pham Ngoc Son, Use of Vietnamese seaweed for functional food, medicine and biofertilizer, Journal Applied Phycology, 2007, Vol. 19, No. 6, pp. 817-826.
[3] Lingchong Wang, Xiangyu Wang, Hao Wu and Rui Liu, Overview on Biological Activities and Molecular Characteristics of Sulfated Polysaccharides from Marine Green Algae in Recent Years, Mar. Drugs, 2014, Vol. 12, pp. 4984-5020.
[4] Hua Tian, Haifang Liu, Weikang Song, Li Zhu & Xueqiong Yin, Polysaccharide from Caulerpa lentillifera: extraction optimization with response surface methodology, structure and antioxidant activities, Natural Product Research, 2019, DOI:10.1080/14786419.2019.1700507
[5] Costa, M.S.S.P.; Almeida-Lima, J.; Farias, E.H.C., Biological activities of sulfated polysaccharides from tropical seaweeds, Biomed. Pharmacother., 2010, Vol. 64, pp. 21–28.
[6] Kausik Chattopadhyay, Utpal Adhikari, Patrice Lerouge, Bimalendu Ray, Polysaccharides from Caulerpa racemosa: Purification and structural features, Carbohydrate Polymers, 2007, Vol. 68, No. 3, pp. 407-415.
[7] Quach Thi Minh Thu, Truong Hai Bang, Nguyen Thi Nu, Đang Vu Luong, Bui Minh Ly, Tran Thi Thanh Van and Thanh Thi Thu Thuy, Structural Determination of Ulvan from Green Seaweed Ulva reticulata Collected at Central Coast of Vietnam, Chemistry Letters, 2015, Vol. 44, No. 6, pp. 788-790.
[8] Thi Thu Thuy Thanh, Thi Minh Thu Quach, Thi Nu Nguyen, Dang Vu Luong, Bui Minh Ly and Thi Thanh Van Tran, Structure and cytotoxic activity of ulvan extracted from green seaweed Ulva lactuca, International Journal of Biological Macromolecules, 2016, Vol. 93, pp. 695-703.
[9] Quách Thị Minh Thu, Nguyễn Hải Minh, Đỗ Thị Thanh Xuân, Đặng Vũ Lương, Trần Thị Thanh Vân, Nguyễn Thị Nụ, Thành Thị Thu Thủy, Sulfate polysaccharide chiết tách từ rong lục chi Cheatomorpha: đặc điểm cấu trúc và hoạt tính chống đông tụ máu, Tạp chí Hóa học, 2019, Vol. 57, No 6E1,2, pp. 117-120.
[10] Quách Thị Minh Thu, Đặng Vũ Lương, Đỗ Thị Thanh Xuân, Nguyễn Quang Tâm, Ngô Văn Quang, Trần Thị Thanh Vân và Thành Thị Thu Thủy, Đặc trưng cấu trúc của polysaccharide chiết tách từ rong lục Enteromorpha intestinalis, Tạp chí Hóa học, 2019, Vol. 57, No 6E1, 2, pp. 113-116.
[11] A. M. Staub, “Removeal of Protein-Sevag Method,” Methods in Carbohydrate Chemistry, 1965, Vol. 5, pp. 5-6.
[12] Bilan MI., Grachev AA, Ustuzhanina NE., A highly regular fraction of a fucoidan from the brown seaweed Fucus distichus L, Carbonhydr Res., 2004, Vol. 339, pp. 511-517.
[13] Dodgson KS, Determination of inorganic sulphate in studies on the enzymic and non-enzymic hydrolysis of carbohydrate and other sulphate esters, Biochem J., 1961, Vol. 78, pp. 312-319.
[14] Philip Skehan, Ritsa Storeng, Dominic Scudiero, Anne Monks, James McMahon, David Vistica, Jonathan T. Warren, Heidi Bokesch, Susan Kenney, Michael R. Boyd, New Colorimetric Cytotoxicity Assay for Anticancer-Drug Screening, Journal of the National Cancer Institute, 1990, Vol. 82, No. 13, pp. 1107-1112.
[15] José Ariévilo Gurgel Rodrigues, Edfranck de Sousa Oliveira Vanderlei, Érika Façanha
Bessa, Francisco de Araújo Magalhães, Regina Célia Monteiro de Paula, Vilma Lima
and Norma Maria Barros Benevides, Anticoagulant Activity of a Sulfated Polysaccharide Isolated from the Green Seaweed Caulerpa cupressoides, Brazilian Archives of Biology and Technology, 2011, Vol. 54, No. 4, pp. 691-700.