Optimization of Ultrasound-assisted Extraction of Ulvan from Green Seaweed Ulva lactuca
Main Article Content
Abstract
In this paper, the extraction conditions of ulvan from green seaweed Ulva lactuca by using Ultrasound-assisted extraction method were optimised. Experience was designed using Box-behnken model with experient areas as following: extraction temperature (X1: 50–90 °C), extraction time (X2: 20–40, min) and solvent-to-material ratio (X3: 50/1–70/1, v/w), objective function (Y: extraction yield) was required to get maximum value. The result showed that the optimization conditions for extraction were: extraction temperature 84.28 °C, extraction time 30.59 min with 60.21/1 (v/w) as solvent-to-material ratio, and the expected objective function Y is 22.86% based on dried seaweed weight.
Keywords:
Ulva lactuca, polysaccharide, green seaweed, extraction, ulvan.
References
[1] A. Alves, R. A. Sousa, R. L. Reis, In Vitro Cytotoxicity Assessment of Ulvan, A Polysaccharide Extracted from Green Algae, Phytother, Res, Vol. 27, 2013, pp. 1143-1148.
[2] J. M. Leiro, R. Castro, J. A. Arranz, J. Lamas, Immunomodulating Activities of Acidic Sulphated Polysaccharides Obtained from the Seaweed Ulva rigida C. Agardh, Int, Immunopharmacol, Vol. 7, 2007, pp. 879-888.
[3] M. Tabarsa, S. J. Lee, S. You, Structural Analysis of Immunostimulating Sulfated Polysaccharides from Ulva pertusa, Carbohydr, Res, 2012, Vol. 361, pp. 141-147.
[4] Hela Yaich, Haikel Garna, Souhail Besbes, Michel Paquot, Christophe Blecker, Hamadi Attia, Chemical Composition and Functional Properties of Ulva Lactuca Seaweed Collected in Tunisia, Food Chemistry, Vol. 128, 2011, pp. 895-901.
[5] M. Lahaye, A. Robic, Structure and Functional Properties of Ulvan- a Polysaccharide from Green Seaweeds, Biomacromolecules, Vol. 8, No. 6, 2007, pp. 1765-1774.
[6] A. Alves, S.G.Caridade, J.F. Mano, R.A. Sousa, R.L. Reis, Extraction and Physico-Chemical Characterization of a Versatile Biodegradable Polysaccharide Obtained from Green Algae, Carbohydr., Res, Vol. 345, 2010, pp. 2194-2200.
[7] T. Maricela, V. Mircea, M. Timothy, Ultrasonically Assisted Extraction of Bioactive Principles from Plants and Their Constituents, 1999, http://doi.org/10.1016/S1569-2868(99)80007-2.
[8] C. Chen, Q. Huang, J. C. K. Rengasamy, C. Wan, Extraction Optimization of Antifungal Compounds from Thalictrum Foliolosum DC, Roots, Outh, African Journal of Botany, Vol. 138, 2021, pp. 328-336.
[9] Y. Zou, X. Chen, W. Yang, S. Liu, Response Surface Methodology for Optimization of the Ultrasonic Extraction of Polysaccharides from Codonopsis Pilosula Nannf, Var, Modesta L. T. Shen, Carbohydr Polym, Vol. 84, 2011, pp. 503-508.
[10] M. G. Vaquero, G. Rajauria, B. Tiwari, Torres Sweeney and John O’Doherty, Extraction and Yield Optimisation of Fucose, Glucans and Associated Antioxidant Activities from Laminaria Digitata by Applying Response Surface Methodology to High Intensity Ultrasound-Assisted Extraction, Mar, Drugs, Vol. 16, 2018, pp. 257-172.
[11] C. Y. Gan, A. A. Latiff, Optimisation of the Solvent Extraction of Bioactive Compounds from Parkia Speciosa Pod Using Response Surface Methodology, Food Chem, Vol. 124, 2011, pp. 1277-1283.
[12] W. H. Mao, L. J. Han, B. Shi, Optimization of Microwave-assisted Extraction of Flavonoid from Radix Astragali Using Response Surface Methodology, Sep, Sci. Technol, Vol. 43, 2008, pp. 671-681.
[13] F. Rahimi, M. Tabarsa, M. Rezaei, Ulvan from Green Algae Ulva intestinalis: Optimization of Ultrasound-assisted Extraction and Antioxidant Activity, Journal of Applied Phycology, Vol. 28, 2016, pp. 2979-2990.
[14] T. T. T. Thuy, Q. T. M Thu, N. T. Nu, D. V. Luong, B. M. Ly, T. T.T. Van, Structure and Cytotoxic Activity of Ulvan Extracted from Green Seaweed Ulva lactuca, International Journal of Biological Macromolecules, Vol. 93,
2016, pp. 695-702.
[2] J. M. Leiro, R. Castro, J. A. Arranz, J. Lamas, Immunomodulating Activities of Acidic Sulphated Polysaccharides Obtained from the Seaweed Ulva rigida C. Agardh, Int, Immunopharmacol, Vol. 7, 2007, pp. 879-888.
[3] M. Tabarsa, S. J. Lee, S. You, Structural Analysis of Immunostimulating Sulfated Polysaccharides from Ulva pertusa, Carbohydr, Res, 2012, Vol. 361, pp. 141-147.
[4] Hela Yaich, Haikel Garna, Souhail Besbes, Michel Paquot, Christophe Blecker, Hamadi Attia, Chemical Composition and Functional Properties of Ulva Lactuca Seaweed Collected in Tunisia, Food Chemistry, Vol. 128, 2011, pp. 895-901.
[5] M. Lahaye, A. Robic, Structure and Functional Properties of Ulvan- a Polysaccharide from Green Seaweeds, Biomacromolecules, Vol. 8, No. 6, 2007, pp. 1765-1774.
[6] A. Alves, S.G.Caridade, J.F. Mano, R.A. Sousa, R.L. Reis, Extraction and Physico-Chemical Characterization of a Versatile Biodegradable Polysaccharide Obtained from Green Algae, Carbohydr., Res, Vol. 345, 2010, pp. 2194-2200.
[7] T. Maricela, V. Mircea, M. Timothy, Ultrasonically Assisted Extraction of Bioactive Principles from Plants and Their Constituents, 1999, http://doi.org/10.1016/S1569-2868(99)80007-2.
[8] C. Chen, Q. Huang, J. C. K. Rengasamy, C. Wan, Extraction Optimization of Antifungal Compounds from Thalictrum Foliolosum DC, Roots, Outh, African Journal of Botany, Vol. 138, 2021, pp. 328-336.
[9] Y. Zou, X. Chen, W. Yang, S. Liu, Response Surface Methodology for Optimization of the Ultrasonic Extraction of Polysaccharides from Codonopsis Pilosula Nannf, Var, Modesta L. T. Shen, Carbohydr Polym, Vol. 84, 2011, pp. 503-508.
[10] M. G. Vaquero, G. Rajauria, B. Tiwari, Torres Sweeney and John O’Doherty, Extraction and Yield Optimisation of Fucose, Glucans and Associated Antioxidant Activities from Laminaria Digitata by Applying Response Surface Methodology to High Intensity Ultrasound-Assisted Extraction, Mar, Drugs, Vol. 16, 2018, pp. 257-172.
[11] C. Y. Gan, A. A. Latiff, Optimisation of the Solvent Extraction of Bioactive Compounds from Parkia Speciosa Pod Using Response Surface Methodology, Food Chem, Vol. 124, 2011, pp. 1277-1283.
[12] W. H. Mao, L. J. Han, B. Shi, Optimization of Microwave-assisted Extraction of Flavonoid from Radix Astragali Using Response Surface Methodology, Sep, Sci. Technol, Vol. 43, 2008, pp. 671-681.
[13] F. Rahimi, M. Tabarsa, M. Rezaei, Ulvan from Green Algae Ulva intestinalis: Optimization of Ultrasound-assisted Extraction and Antioxidant Activity, Journal of Applied Phycology, Vol. 28, 2016, pp. 2979-2990.
[14] T. T. T. Thuy, Q. T. M Thu, N. T. Nu, D. V. Luong, B. M. Ly, T. T.T. Van, Structure and Cytotoxic Activity of Ulvan Extracted from Green Seaweed Ulva lactuca, International Journal of Biological Macromolecules, Vol. 93,
2016, pp. 695-702.