Expression of some Serine Protease Encoding Genes and Trypsin-like Protease Activity in Black Tiger Shrimp (Penaeus monodon) Infected with White Spot Syndrome Virus
Main Article Content
Abstract
In this study, trypsin-like protease activity and expression of serine protease encoding genes (PmPPAE1, PmPPAE2 - clip-serine proteases and PmTLP1, PmTLP2 - trypsin-like proteases) in the different tissues of black tiger shrimp were determined. The expression level of PmPPAE1 and PmPPAE2 was the highest in the hemolymph; meanwhile that of PmTLP1 and PmTLP2 was the highest in the hepatopancreas. In the hemolymph of white spot syndrome virus (WSSV)-injected shrimp, the expression of PmPPAE1, PmPPAE2 and PmTLP2 genes was upregulated by 2.32, 2.57 and 4.56-folds, respectively, whereas the expression of PmTLP1 was downregulated by 1.47-folds relative to the control group (injected with phosphate buffered saline). In the hepatopancreas of WSSV-injected shrimp, the expression levels of PmPPAE1, PmPPAE2 increased by 2.24 and 3.99-folds, respectively, and that of PmTLP1 and PmTLP2 decreased by 1.51, 1.29-folds, respectively. Trypsin-like protease activity was found increased 3.25-fold and decreased 4.1-fold, in the hemolymph and hepatopancreas of WSSV-infected shrimp, respectively. These data indicate the role of serine proteases in immune response of the shrimp.
Keywords:
Clip-serine protease, Penaeus monodon, serine protease, trypsin-like protease, WSSV.
References
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[3] X. Z. Shi, Q. Ren, X. F. Zhao, J. X. Wang, Expression of four trypsin-like serine proteases from the Chinese shrimp, Fenneropenaeus chinensis, as regulated by pathogenic infection, Comparative Biochemistry and Physiology, Vol. 153, No. 1, 2009, pp. 2-3, https://doi.org/10.1016/j.cbpb.2009.01.011.
[4] O. Carrillo-Farnés, A. Forrellat-Barrios, S. Guerrero-Galván & F. Vega-Villasate, A review of digestive enzyme activity in Penaeid shrimps, Crustaceana, Vol. 8, No. 3, 2007, pp. 257–275, https://doi.org/10.1163/156854007780162424.
[5] W. Charoensapsri, P. Amparyupa, I. Hironoc, T. Aoki, A. Tassanakajona, Gene silencing of a prophenoloxidase activating enzyme in the shrimp, Penaeus monodon, increases susceptibility to Vibrio harveyi infection, Developmental and Comparative Immunology, Vol. 33, No. 7, 2009, pp. 815-816, https://doi.org/doi: 10.1016/j.dci.2009.01.006.
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[9] D. Lemos, J. M. Ezquerra & F. L. Garcia-Carreno, Protein digestion in penaeid shrimp: digestive proteinases, proteinase inhibitors and feed digestibility, Aquaculture, Vo. 186, No. 1-2, 2000, pp. 89-105, https://doi.org/10.1016/S0044-8486(99)00371-3.
[10] J. C. Sainz, F. L. García-Carreño & P. Hernández-Cortés, Penaeus vannamei isotrypsins: purification and characterization, Comparative Biochemistry and Physiology, Part B, Vol. 138, No. 2, 2004, pp. 155–162, https://doi.org/10.1016/j.cbpc.2004.03.002.
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[14] A. Sánchez-Paza, F. Fernando García-Carreña, A. Muhlia-Almazána, N. Y. Hernández-Saavedraa, G. Yepiz-Plascencia, Differential expression of trypsin mRNA in the white shrimp (Penaeus vannamei) midgut gland under starvation conditions, Journal of Experimental Marine Biology and Ecology, Vol. 292, No. 1, 2003, pp. 1-17, https://doi.org/10.1016/S0022-0981(03)00142-4.
[15] C. Li, F. Wang, J. J. Aweya, D. Yao, Z. Zheng, H. Huang, S. Li & Y. Zhang, Trypsin of Litopenaeus vannamei is required for the generation of hemocyanin-derived peptides, Development and Comparative Immunology, Vol. 79, 2018), pp. 95–104, https://doi.org/10.1016/j.dci.2017.10.015.
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[17] D. Grenier, Inactivation of human serum bactericidal activity by a trypsin-like protease isolated from Porphyromonas gingivalis, Infection and immunity, Vol. 60, No. 5, 1992, pp. 1854-1857, https://doi.org/10.1128%2Fiai.60.5.1854-1857.1992.
[18] T. H. Ma, J. A. Benzie, J. G. He, C. B. Sun, S. F, Chan, PmPPAF is a pro-phenoloxidase activating factor involved in innate immunity response of the shrimp Penaeus monodon, Developmental and comparative immunology, Vol. 44, No. 1, 2014, pp. 167-168, https://doi.org/10.1016/j.dci.2013.12.007.
[19] S. A. Lehnert & S. E. Johnson, Expression of hemocyanin and digestive enzyme messenger RNAs in the hepatopancreas of the black tiger shrimp Penaeus monodon, Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, Vol. 133, No. 2, 2002, pp. 163-171,
https://doi.org/10.1016/S1096-4959(02)00123-9.