Mitochondrial DNA Copy Number in Colorectal Cancer in Vietnamese Patients
Main Article Content
Abstract
Mitochondria are organelles present in most eukaryotic cells, have their own genome and replicate independently of the nuclear genome. Alterations of mitochondrial DNA (mtDNA) have been shown to be associated with cancer. In particular, each cell has many mtDNA copies, the copy number depends on the cell type and the pathological state of the cell. To understand the role of mtDNA copy number in colorectal cancer (CRC), we quantified mtDNA copy number using real-time PCR method on 115 pairs of cancer tissue samples and 40 blood samples of CRC patients which were provided by hospital K, Military Hospital 103 and 67 blood samples of healthy people were used as controls. The results showed that, on tissue samples, most patients had mtDNA copy numbers in the range of 500-1500. The mtDNA copy number in the tissue sample of colon cancer patients (median: 1487.90) was higher than that of rectal cancer patients (median: 694.84) (p<0.05). The mtDNA copy number tends to increase with age, women are higher than men, and decreases with lymph node metastasis and cancer stage. The mtDNA copy number of blood samples from cancer patients is 3.4 times higher than that of blood samples from healthy people (p<0.05). The correlation of mtDNA copy number between tissue and blood samples from the same cancer patient is weak.
Keywords:
Mitochondrial DNA, mtDNA copy number, colorectal cancer, real-time PCR.
References
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[10] T. Chen, J. He, L. Shen, H. Li et al., The Mitochondrial DNA 4,977-bp Deletion and its Implication in Copy Number Alteration in Colorectal Cancer, BMC Med Genet, Vol. 12, No. 2, 2011, pp. 8-17, https://doi.org/10.1186/ 1471-2350-12-8.
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[17] H. D. Hosgood, C. S. Liu, N. Rothman, D. Albanes et al., Mitochondrial DNA Copy Number and Lung Cancer Risk in A Prospective Cohort Study, Carcinogenesis, Vol. 31, No. 5, 2010, pp. 847-859, https://doi.org/10.1371/ journal.pone.0065968.
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[20] E. Mambo, A. Chatterjee, G. Tallini, S. Sukumar et al., Tumor-Specific Changes in mtDNA Content in Human Cancer, Int J Cancer, Vol. 116, No. 6, 2005, pp. 920-924, https://doi.org/10.1002/ ijc.21110.
[21] A. M. Mohideen, E. Dicks, P. Parfrey, R. Green et al., Mitochondrial DNA Polymorphisms, its Copy Number Change and Outcome in Colorectal Cancer, BMC Res Notes, Vol. 8, No.5, 2015, pp. 272-281, https://doi.org/10.1186/s13104-015-1250-5.
[22] P. Xia, H. X. An, C. X. Dang, W. Holzgreve et al., Decreased Mitochondrial DNA Content in Blood Samples of Patients with Stage Breast Cancer, BMC Cancer, Vol. 9, No. 4, 2009, pp. 454-465, https://doi.org/10.1186/1471-2407-9-454.
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[24] S. D. Maria Angela, C. Dani, Silmara, P. G. Lima, F. Soares et al., Less ∆mtdna4977 than Normal in Various Types of Tumors Suggests that Cancer Cells Are Essentially Free of this Mutation, Genetics and Molecular Research, Vol. 3, No. 3, 2004, pp. 395-409.
[25] H. Nie, G. Chen, J. He, J. Lyu et al., Mitochondrial Common Deletion Is Elevated in Blood of Breast Cancer Patients Mediated by Oxidative Stress, Mitochondrion, Vol. 26, No. 4, 2016, pp. 104-118, https://doi.org/10.1016/j.mito. 2015.12.001.
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