Impact of a Vibrio parahaemolyticus Strain Carrying a Quorum Sensing Inhibiting Gene Construct on the Vitality and Resistance of Whiteleg Shrimp to the Wild-type Strain
Main Article Content
Abstract
Vibrio parahaemolyticus is a widespread pathogen of marine aquaculture animals, especially whiteleg shrimp (Litopenaeus vannamei), with mortality rates of up to 100%, causing substantial economic losses to the marine aquaculture industries. Traditional treatments, including antibiotics and chemicals, are becoming ineffective due to emerging bacterial resistance and concerns related to environmental contamination and human health. Thus, novel approaches for the treatment and prevention of diseases caused by Vibrio bacteria are needed. A recent new and promising approach is to inhibit quorum sensing, which is the regulation of gene expression and thus cell behaviors in response to fluctuations in cell-population density. In this study, we explored whether a V. parahaemolyticus strain with inhibited quorum sensing could express a reduced pathogenicity level and stimulate better resistance in whiteleg shrimp. The strain was
V. parahaemolyticus PBN, which was created in a previous study and harbored a plasmid (pCR2.1) inserted with the gene qrr_vibrio, containing the preservative sequences of quorum-sensing regulator sRNAs (qrr) in Vibrio bacteria. This strain exhibited a diminished capacity for biofilm formation compared to its wild-type counterpart. Furthermore, shrimp infected with PBN demonstrated 20-25% higher survival rates than those exposed to the wild-type strain. We further investigated the impact of various cell densities of PBN on shrimp resistance and found that the resistance of shrimp to the wild-type was enhanced if shrimp were pretreated with higher densities of PBN, particularly at 2×108 and 4×108 CFU/mL. The results are interesting and strongly support the interruption of quorum sensing as an effective approach to control diseases caused by Vibiro bacteria.
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https://doi.org/10.1016/j.aquaculture.2019.734227.
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