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Abstract: Through data synthesis and insilico screening, 21 potential tyrosinase enzyme inhibiting compounds were identified. Specifically, Morus alba was identified as a herb containing tyrosinase enzyme inhibiting substances that could be used in skin whiteners. From Morus alba leaves, the following 11 flavonoid-terpenoid compounds were extracted and isolated: 3, 4-dihydroxybenzoic acid, ellagic acid, gallic acid, Kaempferol 3-O-β-D-glucopyranoside, Quercetin 3-O-α-L-rhamnopyranoside, isofraxidin-7-O-β-D-glucopyranoside, (±) -3, 5, 6, 7, 8, 4'-hexahydroxyflavane, Kaempferol-3, 7-O-α-L-rhamnopyranoside, 7, 4'-dihydroxy-5,3'-dimethoxyflavone, (S) -5, 5', 7-trihydroxy-2', and 4'-dimethoxy-6-methylflavanone. Acute toxicity, skin whitening effects on in vitro and in vivo with extracted fractions and isolated compounds were evaluated. The results show that the high safety fractions and compounds had good whitening effect. Based on the developed product recipe, pilot products in the form of capsule and cream were made and their skin whitening and skin irritant effects were evaluated. The results show that the two products have high safety, good skin whitening effect with no irritation. As a result, food supplement Morus capsules and MELAGENN cosmetics cream have been registered.
Keywords: Morus alba, tyrosinase, insilico, flavonoid, terpenoid.
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