Fabrication of SERS Substrates Using Ag/SiO₂ Nanocomposite for Dectecting Sub-ppm Glucose Concentration
Main Article Content
Abstract
This work presents the fabrication of an Ag/SiO₂ nanocomposite (NC) using the Stöber method coupled with chemical reduction, which serves as an effective SERS platform for the ultrasensitive detection of glucose at sub-ppm concentrations. SEM analysis revealed that the SiO₂ is in the form of nanospheres (NSs) with an average diameter of 159 nm, while TEM images showed Ag nanoparticles (NPs) with an average diameter of approximately 17 nm, uniformly distributed on the SiO₂ surface. Such a well-defined nanostructure optimizes the SERS performance by providing distinct Raman signals corresponding to the functional groups in glucose molecules. In this study, glucose was successfully detected in the concentration range from 20 ppm down to 1 ppm, with a characteristic Raman peak observed at 1334 cm⁻¹.
Keywords:
Ag/SiO2, Nanocomposite, SERS, D–Glucose
References
[1] D. V. Viazmitinov, L. B. Matyushkin, A. I. Maximov, Synthesis of Core-shell Ag/SiO₂ Nanoparticles for SPASER Structures, Journal of Physics: Conference Series, Vol. 541, No. 1, 2014, pp. 012015, https://doi.org/10.1088/1742-6596/541/1/012015.
[2] S. A. Maier, Plasmonics: Fundamentals and Applications, Springer Science & Business Media, New York, 2007, https://doi.org/10.1007/0-387-37825-1.
[3] M. W. Zhu, G. D. Qian, Z. L. Hong, Z. Y. Wang, X. P. Fan, M. Q. Wang, Preparation and Characterization of Monodisperse Silver Nanoparticles Embedded into Silica Spheres, Journal of Physics and Chemistry of Solids, Vol. 66, 2005, pp. 748-752, https://doi.org/10.1016/j.jpcs.2004.09.013.
[4] X.-F. Zhang, Z.-G. Liu, W. Shen, S. Gurunathan, Silver Nanoparticles: Synthesis, Characterization, Properties, Applications, and Therapeutic Approaches, International Journal of Molecular Sciences, Vol. 17, No. 9, 2016,
pp. 1534, https://doi.org/10.3390/ijms17091534.
[5] X. Sun, S. P. Stagon, H. Huang, J. Chen, Y. Lei, Functionalized Aligned Silver Nanorod Arrays for Glucose Sensing through Surface Enhanced Raman Scattering, RSC Advances, Vol. 4, 2014, pp. 23382-23388, https://doi.org/10.1039/C4RA02423K.
[6] L. Pérez-Mayén, J. Oliva, P. Salas, E. De la Rosa-Cruz, Nanomolar Detection of Glucose Using SERS Substrates Fabricated with Albumin-Cated Gold Nanoparticles, Nanoscale, Vol 8, No. 20, 2016, pp. 10249-10256, https://doi.org/10.1039/C6NR00163G.
[7] N. T. Hue, N.T.Dai , B.H.Van, V.T.Bich , D. V.Thai, N.T.T. Phuong , T.T.Duc , N.T.H. Giang , Q. T. Ha, N. M. Hung, Fabrication of Ag/SiO₂ Nanocomposite via The Stöber and Chemical Reduction Approach for Detecting Sub ppm Tetracycline Concentration, Materials Chemistry and Physics, Vol 341, 2025, pp. 130885, https://doi.org/10.1016/j.matchemphys.2025.130885.
[8] W. Stöber, A. Fink, E. Bohn, Controlled Growth of Monodisperse Silica Spheres in the Micron Size Range, Journal of Colloid and Interface Science, Vol. 26, No. 1, 1968, pp. 62-69, https://doi.org/10.1016/0021-9797(68)90272-5.
[9] I. A. Rahman, V. Padavettan, Synthesis of Silica Nanoparticles by Sol-gel: Size-Dependent Properties, Surface Modification, and Applications in Silica-Polymer Nanocomposites, Journal of Nanomaterials, Vol. 2012, 2012, Article ID 132424, https://doi.org/10.1155/2012/132424.
[10] Z. Li, J. C. Barnes, A. Bosoy, J. F. Stoddart, J. I. Zink, Mesoporous Silica Nanoparticles in Biomedical Applications, Chemical Society Reviews, Vol. 41, No. 7, pp. 2590-2605, https://doi.org/10.1039/C1CS15246G.
[11] S. A. Baker, N. P. M. N. Nagendra, B. L. Dhananjaya, K. M. Mohan, S. Yallappa, S. Satish, Synthesis of Silver Nanoparticles by Endosymbiont Pseudomonas Fluorescens CA 417 and Their Bactericidal Activity, Letters in Applied Microbiology, Vol. 48, No. 3, 2016, pp. 280-286, https://doi.org/10.1016/j.enzmictec.2016.10.004.
[12] J. J. Cael, J. L. Koenig, J. Blackwell, Infrared and Raman Spectroscopy of Carbohydrates. Part IV. Identification of Configuration- and Conformation-Sensitive Modes for D-glucose by Normal Coordinate Analysis, Carbohydrate Research, Vol. 32, 1974, pp. 79-91, http://doi.org/10.1016/S0008-6215(00)82465-9.
[13] X. Sun, Glucose Detection through Surface-enhanced Raman Spectroscopy: A Review, Analytica Chimica Acta, Vol. 1206, 2022, Artical ID 339226, http://doi.org/10.1016/j.aca.2021.339226.
[14] American Diabetes Association, Diagnosis and Classification of Diabetes Mellitus, Diabetes Care, Vol. 33, Supplement. 1, 2010, pp. S62-S69, https://doi.org/10.2337/dc10-S062.
[2] S. A. Maier, Plasmonics: Fundamentals and Applications, Springer Science & Business Media, New York, 2007, https://doi.org/10.1007/0-387-37825-1.
[3] M. W. Zhu, G. D. Qian, Z. L. Hong, Z. Y. Wang, X. P. Fan, M. Q. Wang, Preparation and Characterization of Monodisperse Silver Nanoparticles Embedded into Silica Spheres, Journal of Physics and Chemistry of Solids, Vol. 66, 2005, pp. 748-752, https://doi.org/10.1016/j.jpcs.2004.09.013.
[4] X.-F. Zhang, Z.-G. Liu, W. Shen, S. Gurunathan, Silver Nanoparticles: Synthesis, Characterization, Properties, Applications, and Therapeutic Approaches, International Journal of Molecular Sciences, Vol. 17, No. 9, 2016,
pp. 1534, https://doi.org/10.3390/ijms17091534.
[5] X. Sun, S. P. Stagon, H. Huang, J. Chen, Y. Lei, Functionalized Aligned Silver Nanorod Arrays for Glucose Sensing through Surface Enhanced Raman Scattering, RSC Advances, Vol. 4, 2014, pp. 23382-23388, https://doi.org/10.1039/C4RA02423K.
[6] L. Pérez-Mayén, J. Oliva, P. Salas, E. De la Rosa-Cruz, Nanomolar Detection of Glucose Using SERS Substrates Fabricated with Albumin-Cated Gold Nanoparticles, Nanoscale, Vol 8, No. 20, 2016, pp. 10249-10256, https://doi.org/10.1039/C6NR00163G.
[7] N. T. Hue, N.T.Dai , B.H.Van, V.T.Bich , D. V.Thai, N.T.T. Phuong , T.T.Duc , N.T.H. Giang , Q. T. Ha, N. M. Hung, Fabrication of Ag/SiO₂ Nanocomposite via The Stöber and Chemical Reduction Approach for Detecting Sub ppm Tetracycline Concentration, Materials Chemistry and Physics, Vol 341, 2025, pp. 130885, https://doi.org/10.1016/j.matchemphys.2025.130885.
[8] W. Stöber, A. Fink, E. Bohn, Controlled Growth of Monodisperse Silica Spheres in the Micron Size Range, Journal of Colloid and Interface Science, Vol. 26, No. 1, 1968, pp. 62-69, https://doi.org/10.1016/0021-9797(68)90272-5.
[9] I. A. Rahman, V. Padavettan, Synthesis of Silica Nanoparticles by Sol-gel: Size-Dependent Properties, Surface Modification, and Applications in Silica-Polymer Nanocomposites, Journal of Nanomaterials, Vol. 2012, 2012, Article ID 132424, https://doi.org/10.1155/2012/132424.
[10] Z. Li, J. C. Barnes, A. Bosoy, J. F. Stoddart, J. I. Zink, Mesoporous Silica Nanoparticles in Biomedical Applications, Chemical Society Reviews, Vol. 41, No. 7, pp. 2590-2605, https://doi.org/10.1039/C1CS15246G.
[11] S. A. Baker, N. P. M. N. Nagendra, B. L. Dhananjaya, K. M. Mohan, S. Yallappa, S. Satish, Synthesis of Silver Nanoparticles by Endosymbiont Pseudomonas Fluorescens CA 417 and Their Bactericidal Activity, Letters in Applied Microbiology, Vol. 48, No. 3, 2016, pp. 280-286, https://doi.org/10.1016/j.enzmictec.2016.10.004.
[12] J. J. Cael, J. L. Koenig, J. Blackwell, Infrared and Raman Spectroscopy of Carbohydrates. Part IV. Identification of Configuration- and Conformation-Sensitive Modes for D-glucose by Normal Coordinate Analysis, Carbohydrate Research, Vol. 32, 1974, pp. 79-91, http://doi.org/10.1016/S0008-6215(00)82465-9.
[13] X. Sun, Glucose Detection through Surface-enhanced Raman Spectroscopy: A Review, Analytica Chimica Acta, Vol. 1206, 2022, Artical ID 339226, http://doi.org/10.1016/j.aca.2021.339226.
[14] American Diabetes Association, Diagnosis and Classification of Diabetes Mellitus, Diabetes Care, Vol. 33, Supplement. 1, 2010, pp. S62-S69, https://doi.org/10.2337/dc10-S062.