Fast DNA Diagnostic Using Fe3O4 Magnetic Nanoparticles and Light Emitting ZnS/Mn Nanoparticles
Main Article Content
Abstract
Abstract: In this study, the fast DNA diagnostics was successfully developed using high fluorescent Mn doped ZnS nanoparticles and NH2-modified SiO2 coated Fe3O4 nanoparticles in a sandwich structure. In one end of the sandwich structure, we employed NH2-modified SiO2 coated Fe3O4 nanoparticles as a docking matrix. A so-called capture probe oligonucleotide chain, that specifically identifies the target DNA, linked to the docking magnetic particles. In the other end, other oligonucleotide chain – named detector probe – contacted with the signaling semiconductor particles. The complementary hybridization of the detector probe – target – capture probe formed the sandwich configuration that attached the fluorescent particles to the docking matrix. This configuration was used to detect DNA of Epstein-Bar virus (EBV) and as the result, a fine affinity between the luminescent intensity at 586 nm of Mn doped ZnS colloids showed with the initially added DNA target concentration, which could detect the presence of target DNA within 2×106 copies/ml (~0.3 fM). The mobility of the as-prepared solutions used in this methodology promised an attractive applicability for designing a DNA detecting fast KIT.
Keywords: DNA fast kit, ZnS nanoparticles, Fe3O4 nanoparticles, Fluorescent.References
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[2] Lijia Shao, Yanfang Gao and Feng Yan, Semiconductor quantum dots for biomedical applications, Sensor 11(2011), 11736-11751.
[3] Xiao Gao, Lily Yang, John A Petros, Fray F Mashall, Jonathan W Simons and Shuming Nie, In vivo molecular and cellular imaging with quantum dots, Current Opinion in Biotechnology 16 (2005), 63-72.
[4] Andrew M. Smith, Hongwei Duan, Aaron M. Mohs and Shuming Nie, Bioconjugated quantum dots for in vivo molecular and cellular imaging, Adv. Drug. Deliv Rev. 60(11) (2008), 1226-1240.
[5] Brad A. Kairdolf, Andrew M. Smith, Todd H. Stokes, May D. Wang, Andrew N. Young and Shuming Nie, Semiconductor quantum dots for bioimaging and biodiagnostic applications, Annu Rev Anal Chem. 6(1) (2013), 143-162.
[6] Igor L. Medintz, H. Tetsuo Uyeda, Ellen R. Goldman and Hedi Mattoussi, Quantum dot bioconjugates for imaging, labeling and sensing, Nature Materials 4(2005), 435-446.
[7] Takashi Jin, Akira Sasaki, Masataka Kinjo and Jun Myazaki, Quantum dot-based ratiometric pH sensor, Chemical Communications 46 (2010), 2408-2410.
[8] Manuela F. Frasco and Nikos Chaniotakis, Semiconductor quantum dots in chemical sensors and biosensors, Sensors 9(2009), 7266-7286.
[9] Wei Lian, Sally A. Litherland, Hassan Badrane, Weihong Tan, Donghai Wu, Henry V. Baker, Paul A. Gulig, Daniel V. Lim, Shouguang Jin, Ultrasensitive detection of biomolecules with fluorescent dye-doped nanoparticles, Analitical biochemistry 334 (2004),135-144.
[10] Jiang Zhengran, Li Ruiyun, Todd Nevins W., Stass Sanford A., Jiang Feng, Detecting genomic aberrations by fluorescence in situ hybridization with quantum dots-labeled probes, Journal of Nanoscience and Nanotechnology 12 (2007), 4254-4259.
[11] Cheng Chi, Fernando Vargas-Lara, Alexei V. Tkachenko, Francis W. Starr and Oleg Gang, Internal structure of nanoparticle dimers linked by DNA, American Chemical Society 6(8) (2012), 6793-6802.
[12] Vaishali Bagalkot, Liangfang Zhang, Etgar Levy-Nissenbaum, Sangyong Jon, Philip W. Kantoff, Robert Langer and Omid C. Farokhzad, Quantum dot-aptamer conjugates for synchronous cancer imaging, therapy, and sensing of drug delivery based on bi-fluorescence resonance energy transfer, Nano Letter 7(10) (2007), 3065-3070.
[13] Ram Kripal, Atul K. Gupta, Sheo K. Mishra, Rajneesh K. Srivastava, Avinash C. Pandey, S.G. Prakash, Photoluminescence and photoconductivity of ZnS:Mn2+ nanopaticles synthesized via co-precipitation method, Spectrochimica Acta Part A 76 (2010), 523-530.
[14] H.C. Warad. S.C. Ghosh, B. Hemtanon, C. Thananchayanont, J. Dutta, Luminescent nanopaticles of Mn doped ZnS passivated with soldium hexametaphosphate, Science and Technology of Advanced Materials 6(2005), 296-301.
[15] Tran Thi Quynh Hoa, Ngo Duc The, Stephen McVitie, Nguyen Hoang Nam, Le Van Vu, Ta Dinh Canh and Nguyen Ngoc Long, Optical properties of Mn-doped ZnS semiconductor nanoclusters synthesized by a hydrothermal process. Optical Materials 33(3) (2011), 308-314.
[16] Dongyeon Son, Dae-Ryong Jung, Jongmin Kim, Taeho Moon, Chunjoong Kim and Byungwoo Park, Synthesis and photoluminescence of Mn-doped zinc sulfide nanoparticles, Applied Physics Letter 9 (2007) 0, 101910.
[17] Yu Z, Ma X, Yu B, Pan Y, Liu Z, Synthesis and characterization of ZnS:Mn/ZnS core/shell nanoparticles for tumor targeting and imaging in vivo, J. Biomater Appl. 28(2) (2013), 232-240.
[18] Tran Thi Quynh Hoa, Le Thi Thanh Binh, Le Van Vu, Nguyen Ngoc Long, Vu Thi Hong Hanh, Vu Duc Chinh, Pham Thu Nga, Luminescent ZnS:Mn/thioglycerol and ZnS:Mn/ZnS core/shell nanocrystals: synthesis and characterization, Optical Materials 35(2) (2012), 136-140.
Dmitry Zimnisky, Chaoyang Jiang, Jun Xu, Zhiqun Lin anf Vladimir V. Tsukruk, Substrate and time-dependent photoluminescence of quantum dots inside the ultrathin polymer LbL film, Langmuir 23 (2007), 4509-4515.
[19] Tian Long Zhang, yun Sheng Xia, Xue Lian Diao, Chang Quing Zhu, Preparation and formation mechanism of strong violet luminescent CdS quantum dots by using a ligand exchange strategy, Journal of Nanoparticles Research 10(1) (2008), 59-67.
[20] Goutam Palui, Tommaso Avellini, Naiquain Zhan, Feng Pan, David Gray, Igor Alabugin and Hedi Matoussi, Photoinduced phase transfer of luminescent quantum dots for polar and aqueous media, J.A.C.S. 134(39) (2012), 16370-16378.
[21] Dao Van Quy, Nguyen Minh Hieu, Pham Thi Tra, Nguyen Hoang Nam, Nguyen Hoang Hai, Nguyen Thai Son, Phan Tuan Nghia, Nguyen Thi Van Anh, Tran Thi Hong and Nguyen Hoang Luong, Synthesis of silica-coated magnetic nanoparticles and application in the detection of pathogenic viruses, Journal of Nanomaterials 2013 ID. 063940.
[22] Jerina Majeed, Lina Pradhan, Raghumani Singh Ningthoujam, Rajesh Kumar Vatsa, Dhirendra Bahadur, Avesh Kumar Tyagi, Enhanced specific absorption rate in silanol functioncalized Fe3O4 core-shell nanoparticles: study of Fe leaching in Fe3O4 and hyperthermia in L929 and HeLa cells, Colloids and Surface B 122 (2014), 396-403.
[23] Nathalie Zammatteo, Laurent Jeanmart, Sandrine Hamels, Stephane Courtois, Pierre Louette, Laszlo Hevesi and Jose Remacle, Comparison between different strategies of covalent attachment of DNA to glass surfaces to build DNA microarray, Analitical Biochemistry 28 (2000), 143-150.
[24] Nguyen Hoang Hai, Nguyen Chau, Nguyen Hoang Luong, Nguyen Thi Van Anh, Phan Tuan Nghia, Applications of magnetic nanoparticles for water treatment and for DNA and cell separation. Journal of the Korean Physical Society 53(2008), 1601-1606.
[25] Nguyen Thuy Trang, Luu Manh Quynh, Tran Van Nam and Hoang Nam Nhat, Charge transfer at organic–inorganic interface of surface-activated PbS by DFT method, Surface Science 608(2013), 67-73.