Le Quynh Mai, Ha Thi Hang

Main Article Content


Abstract: Salinity is one of the abiotic stresses that reduces the growth and development of plant. Soybean (Glycine max [L.] Merr.) is known to be sensitive to salinity; not only agronomy traits but also nodulation of soybean plant are inhibited in high salt concentration, thus reduce the yield of soybean. To cope with salt stress, soybean has developed several tolerance mechanisms. One of those is accumulation of comparative solutes which induce high osmotic potential for plant cells. Proline considered as a comparative solute was reported to play a critical role in increasing salt tolerance. However,  knowledge about salt acclimation, the phenomenon of increase salt tolerance after exposing to salt stress at lower level before, are limited. Here, the changes of proline during salt acclimation in germination stage of soybean DT26 variety were studied. Proline content of salt acclimation and non-acclimation samples were compared to find out the role of acclimation in inducing salt tolerance in soybean through accumulation of proline. The results indicated the actually enhancement of proline biosynthesis during salt acclimation but it really differed from tissue to tissue of soybean plant.

Keywords: Proline, salt acclimation, salt tolerance, soybean, Glycine max, DT26.


[1] Kazem G.-G. and Minoo T. -N., Soybean performance under salinity stress, in: Prof. Tzi-Bun Ng (Ed.) Soybean - Biochemistry, Chemistry and Physiology, ISBN: 978-953-307-219-7, InTech (2011): 631-642.
[2] Türkan I. and Demiral T., Recent developments in understanding salinity tolerance, Environmental and Experimental Botany 67 (2009): 2 – 9.
[3] Munns R., Tester M., Mechanisms of salt tolerance, Annual Review Plant Biology 59 (2008): 651- 681.
[4] Phang T. -H., Shao G. and Lam H. -M., Salt Tolerance in Soybean, Journal of Integrative Plant Biology 50(10) (2008): 1196–1212.
[5] Aziz A., Martin-Tanguy J., Larher F., Salt stress-induced proline accumulation and changes in tyramine and polyamine levels are linked to ionic adjustment in tomato leaf discs, Plant Science 145 (1999): 83 - 91.
[6] Watanabe A., Kojima K., Ide Y., Sasaki S., Effects of saline and osmotic stress on proline and sugar accumulation in Populus euphratica in vitro, Plant Cell Tissue & Organ Culture 63 (2000): 199-206.
[7] Ashraf M., Some important physiological selection criteria for salt tolerance in plants, Flora 199 (2004): 361 - 376.
[8] Liu J. and Zhu J.-K., Proline accumulation and salt-stress-induced gene expression in a salt-hypersensitive mutant of Arabidopsis, Plant Physiology 114 (1997): 591 - 596.
[9] Ashraf M. and Harris P., Potential biochemical indicators of salinity tolerance in plants, Plant Science 166 (2004): 3 - 16.
[10] Chen P., Yan K., Shao H., Zhao S., Physiological mechanisms for high salt tolerance in wild soybean (Glycine soja) from Yellow River Delta, China: photosynthesis, osmotic regulation, ion flux and antioxidant capacity, (2013), PloS ONE 8(12): e83227. Doi:10.1371/journal.pone.0083227.
[11] Chen Z., Cuin T.A., Zhou M., Twomey A., Naidu B.P., Shabala S., Compatible solute accumulation and stress mitigating effects in barley genotypes contrasting in their salt tolerance, Journal of Experimental Botany 58 (2007): 4245 – 4255.
[12] Kondetti P., Jawali N., Apte S. K. and Shitole M.G., Salt tolerance in Indian soybean (Glycine max (L.) Merill) varieties at germination and early seedling growth, Annals of Biological Research 3(3) (2012): 1489-1498.
[13] Hoagland D.R., The water-culture method for growing plants without soil, in: Circular (California Agricultural Experiment Station, 347. Ed.), Berkeley, Calif.: University of California, College of Agriculture, Agricultural Experiment Station (1938).
[14] Hoagland D. R. and Arnon D.O., The water-culture method for growing plants without soil, Berkeley, Calif.: University of California, College of Agriculture, Agricultural Experiment Station (1950).
[15] Kabir M.H., Islam M.M., Begum S.N. and Manidas A.C., Application of SSR technique for the identification of markers linked to salinity tolerance in rice, Progress. Agric. 19(2) (2008): 57 - 65.
[16] Bates L.S., Waldren R.P., Teare I.D., Rapid determination of free proline for water-stress studies, Plant Soil 39 (1973): 205 - 207.
Klaus H. and Oscar K., Design and Analysis of Experiments I and II (Second ed.), Wiley. ISBN 978-0-470-38551-7 (2008).