Genetic Polymorphism in Promoter Sequence of CYP2E1 Gene in Paint Manufacturing Workers Exposed to Volatile Organic Compounds

Abstract

Abstract: Enzyme CYP2E1 plays an important role in the metabolism and bio-activation of volatile organic compounds (VOCs) such as benzene, toluene, xylene (B, T, X), commonly used in the industry. The presence of the polymorphism in the promoter region of CYP2E1 gene may lead to changes in gene expression and the progression of some diseases in people exposed to VOCs. This study analyzes the polymorphism of CYP2E1 promoter which can affect the expression of this gene and is related to the accumulation of toxic products in 118 participants including 73 workers of the exposed group from paint manufacturing factories and 45 workers of the non-exposed group from garment factories by using Polymerase Chain Reaction and DNA sequencing methods. The results show that the expected DNA fragment was amplified in all the samples and 4 polymorphic 3739G>C, 3620C>T, 3519T>G, and 3468T>A were observed. The relationship between these SNPs of CYP2E1 and the exposure of VOCs is not shown. However, there was heterogeneity in CYP2E1 genotype among the studied subjects.

Keywords: Single nucleotide polymorphism, CYP2E1, metabolism of volatile organic compounds.

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