Nguyen Thi Hien, Nguyen Thi Cam Nhung, Nguyen Phu Hung, Nguyen Thi Hong Van, Bui Phuong Thuan, Nguyen Quang Huy

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Abstract: Enzyme CYP2E1 plays an important role in the metabolism and bio-activation of volatile organic compounds (VOCs) such as benzene, toluene, xylene (B, T, X), commonly used in the industry. The presence of the polymorphism in the promoter region of CYP2E1 gene may lead to changes in gene expression and the progression of some diseases in people exposed to VOCs. This study analyzes the polymorphism of CYP2E1 promoter which can affect the expression of this gene and is related to the accumulation of toxic products in 118 participants including 73 workers of the exposed group from paint manufacturing factories and 45 workers of the non-exposed group from garment factories by using Polymerase Chain Reaction and DNA sequencing methods. The results show that the expected DNA fragment was amplified in all the samples and 4 polymorphic 3739G>C, 3620C>T, 3519T>G, and 3468T>A were observed. The relationship between these SNPs of CYP2E1 and the exposure of VOCs is not shown. However, there was heterogeneity in CYP2E1 genotype among the studied subjects.

Keywords: Single nucleotide polymorphism, CYP2E1, metabolism of volatile organic compounds.


[1] Junko W., Hayashi S., Kaname K. (1994), Different Regulation and Expression of the Human CYP2E1 Gene Due to the Rsal Polymorphism in the 5-Flanking, J. Biochem,116, 321-326.
[2] Peter R., Bocker R., Beaune P.H., Iwasaki M., Guengerich F.P., et al. (1990), Hydroxylation of chlorzoxazone as a specific probe for human liver cytochrome P 450IIE1, Chem Res Toxicol, 3, 566–573.
[3] Guengerich F.P., Kim D.H., Iwasaki M. (1991), Role of human cytochrome P-450 IIE1 in the oxidation of many low molecular weight cancer suspects, Chem Res Toxicol, 4, 168–179.
[4] Kharasch E.D., Thummel K.E. (1993), Identification of cytochrome P450 2E1 as the predominant enzyme catalyzing human liver microsomal defluorination of sevoflurane, isoflurane, and methoxyflurane, Anesthesiology,79, 795–807.
[5] Lieber C.S. (1997), Cytochrome P-4502E1: its physiological and pathological role,Physiol Rev, 77, 517–544.
[6] Bolt H.M., Roos P.H., Thier R. (2003), The cytochrome P-450 isoenzymeCYP2E1 in the biological processing of industrial chemicals: consequences for occupational and environmental medicine, Int Arch Occup Environ Health,76, 174-185.
[7] Hayashi S., Watanabe J., Kawajiri K. (1991), Genetic polymorphisms in the 5 prime flanking region change transcriptional regulation of the humancytochrome P450IIE1 gene, J. Biochem, 110, 559-565.
[8] Wan J., Shi J., Hui L., Wu D., Jin X., Zhao N., Huang W., Xia Z. (2002),
Association of genetic polymorphisms in CYP2E1, MPO, NQO1, GSTM1, and GSTT1genes with benzene poisoning, Environ. Health Perspect, 110, 1213–1218.
[9] Linhao Z., Yongjun H., Fanglin N. et al. (2018), Polymorphisms of drug-metabolizing enzymeCYP2E1 in Chinese Uygur population, Medicine, 97(7), p. e9970
[11] Chen Y., Li G., Yin S. et al.(2007), Genetic polymorphisms involved in toxicant-metabolizing enzymes and the risk of chronic benzene poisoning in Chinese occupationally exposed populations, Xenobiotica, 37(1), 103-112.
[12] Lan Q., Zhang L., Li G. et al.(2004), Hepatotoxicity in workers exposed to low levels of benzene, Science, 306 (5702), 1774-1776.
[13] Mitri S., Fonseca A.S.A, Otero U.B. et al.(2015), Metabolic polymorphisms and clinical findings related to benzene poisoning detected in exposed Brazilian gas-station workers, Int.J.Environ. Res.Public Health, 12 (7), 8434-8447.
[14] Zhang G.H., Ye L.L., Wang J.W. et al. (2014), Effect of polymorphic metabolizing genes on micronucleus frequencies among benzene-exposed shoe workers in China,Int.J.Environ.Res.Public Health,217(7), 726-732.
[15] Huang X., Chen L., Song W. et al.(2012), Systematic functional characterization of cytochrome P450 2E1 promoter variants in the Chinese Han population, PloS one, 7(7), p. e40883.