Tran Quoc Tuan, Pham Van Hao, Luu Manh Quynh, Nguyen Hoang Luong, Nguyen Hoang Hai

Main Article Content


Silver nanoparticles colloid has been prepared by heat-combined electrochemical method, which uses simple and low-cost equipment of easy installation and operation, is easily deployed in industrial scale. A silver plate was used as the cathode instead of silver salts to avoid unexpected ions from the salts. The cathode was made from a stainless steel plate. The chemical used as the electrolyte solution is TriSodium Citrate (TSC). Silver nanoparticles made by the above method are spherical, small-sized, its size distribution ranges from 3-12nm, stably dispersing in a non-toxic solution.

Bactericidal capacity of silver nanoparticles are tested on 4 types of common bacteria and fungi. The results showed that silver nanoparticles with a concentration of 10 ppm have a good bactericidal capacity against the said bacteria. This result shows that silver nanoparticles made by heat-combined electrochemical method can be used in antibacterial applications.

Keywords: Silvernanoparticle, electrolysis, TriSodium Citrate, antibacterial.


[1] L. Karthik, G. Kumar, A. V. Kirthi, A. Rahuman, and K. B. Rao, Streptomyces sp. LK3 mediated synthesis of silver nanoparticles and its biomedical application. Bioprocess and biosystems engineering, 2014, 37(2), 261-267.
[2] L. Budama, B. A. Çakır, Ö. Topel, and N. Hoda, A new strategy for producing antibacterial textile surfaces using silver nanoparticles. Chemical Engineering Journal, 2013, 228, 489-495.
[3] D. Ren and J. A. Smith, Retention and Transport of Silver Nanoparticles in a Ceramic Porous Medium Used for Point-of-Use Water Treatment. Environmental science & technology, 2013, 47(8), 3825-3832.
[4] P. Kanmani and S. T. Lim, Synthesis and structural characterization of silver nanoparticles using bacterial exopolysaccharide and its antimicrobial activity against food and multidrug resistant pathogens. Process Biochemistry, 2013, 48(7), 1099-1106.
[5] S. Kokura, O. Handa, T. Takagi, T. Ishikawa, Y. Naito, and T. Yoshikawa, Silver nanoparticles as a safe preservative for use in cosmetics. Nanomedicine: Nanotechnology, Biology and Medicine, 2010, 6(4), 570-574.
[6] S. Pal, Y. K. Tak, and J. M. Song, Does the antibacterial activity of silver nanoparticles depend on the shape of the nanoparticle? A study of the gram-negative bacterium Escherichia coli. Applied and environmental microbiology, 2007, 73(6), 1712-1720.
[7] V. K. Sharma, R. A. Yngard, and Y. Lin, Silver nanoparticles: green synthesis and their antimicrobial activities. Advances in colloid and interface science, 2009, 145(1), 83-96.
[8] G. M. Herrera, A. C. Padilla, and S. P. Hernandez-Rivera, Surface Enhanced Raman Scattering (SERS) Studies of Gold and Silver Nanoparticles Prepared by Laser Ablation. Nanomaterials, 2013, 3(1), 158-172.
[9] S. Wang, Y. Zhang, H. -L. Ma, Q. Zhang, W. Xu, J. Peng, J. Li, Z. -Z. Yu, and M. Zhai, Ionic-liquid-assisted facile synthesis of silver nanoparticle-reduced graphene oxide hybrids by gamma irradiation. Carbon, 2013, 55, 245-252.
[10] Z. Jia, H. Sun, and Q. Gu, Preparation of Ag nanoparticles with triethanolamine as reducing agent and their antibacterial property. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 2013, 419, 174-179.
[11] X. Zhao, Y. Xia, Q. Li, X. Ma, F. Quan, C. Geng, and Z. Han, Microwave-assisted synthesis of silver nanoparticles using sodium alginate and their antibacterial activity. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 2014, 444, 180-188.
[12] V. N. Phan, H. Lan, N. T. Thuy, T. M. Hien, T. Q. Huy, N. Van Quy, H. D. Chinh, L. M. Tung, P. A. Tuan, and V. D. Lam, Characterization and antimicrobial activity of silver nanoparticles prepared by a thermal decomposition technique. Applied Physics A, 2013, 113(3), 613-621.
[13] M. Roldán, N. Pellegri, and O. de Sanctis, Electrochemical Method for Ag-PEG Nanoparticles Synthesis. Journal of Nanoparticles, 2013, 2013.
[14] T. Q. Tuan, N. V. Son, H. T. K. Dung, N. H. Luong, B. T. Thuy, N. T. V. Anh, N. D. Hoa, and N. H. Hai, Preparation and properties of silver nanoparticles loaded in activated carbon for biological and environmental applications. Journal of hazardous materials, 2011, 192(3), 1321-1329.
[15] M. T. Reetz and W. Helbig, Size-selective synthesis of nanostructured transition metal clusters. Journal of the American Chemical Society, 1994, 116(16), 7401-7402.
[16] L. Rodriguez-Sanchez, M. Blanco, and M. Lopez-Quintela, Electrochemical synthesis of silver nanoparticles. The Journal of Physical Chemistry B, 2000, 104(41), 9683-9688.
[17] J. McFarland, The nephelometer: an instrument for estimating the number of bacteria in suspensions used for calculating the opsonic index and for vaccines. Journal of the American Medical Association, 1907, 49(14), 1176-1178.
[18] Khaydarov R., R. Khaydarov, O. Gapurova, Y. Estrin, and T. Scheper (2009), Electrochemical method for the synthesis of silver nanoparticles. Journal of Nanoparticle Research. 11(5), pp.1193-1200.
[19] Šileikaitė A., I. Prosyčevas, J. Puišo, A. Juraitis, and A. Guobienė (2006), Analysis of silver nanoparticles produced by chemical reduction of silver salt solution. Mater. Sci.-Medzg. 12, pp.287-291.
[20] S. Link and M. A. El-Sayed, Shape and size dependence of radiative, non-radiative and photothermal properties of gold nanocrystals. International Reviews in Physical Chemistry, 2000, 19(3), 409-453.
[21] J. Y. Kim, C. Lee, M. Cho, and J. Yoon, Enhanced inactivation of< i> E. coli and MS-2 phage by silver ions combined with UV-A and visible light irradiation. Water research, 2008, 42(1), 356-362.
[22] W. -R. Li, X. -B. Xie, Q. -S. Shi, H. -Y. Zeng, O. -Y. You-Sheng, and Y. -B. Chen, Antibacterial activity and mechanism of silver nanoparticles on Escherichia coli. Applied microbiology and biotechnology, 2010, 85(4), 1115-1122.
[23] K. -J. Kim, W. S. Sung, B. K. Suh, S. -K. Moon, J. -S. Choi, J. G. Kim, and D. G. Lee, Antifungal activity and mode of action of silver nano-particles on Candida albicans. Biometals, 2009, 22(2), 235-242.
[24] G. Martinez-Castanon, N. Nino-Martinez, F. Martinez-Gutierrez, J. Martinez-Mendoza, and F. Ruiz, Synthesis and antibacterial activity of silver nanoparticles with different sizes. Journal of Nanoparticle Research, 2008, 10(8), 1343-1348.