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Copper nanodisks (Cu NDs) of 50 nm were prepared on square-inch anodic aluminum oxide substrates by sputtering method. The samples were annealed at 450oC, then the walls of AAO substrates were lift off in a solution of acid phosphoric. The 2D arrays of Cu NDs were fabricated in high quality. Morphology of the substrates were observed by scanning electron microscopy. Surface plasmon resonance absorption was observed with different peaks in the range of 400-1400nm wavelengths that means the substrate is promising for SERS application. To demonstrate, we observed Raman spectrum of Rhodamine 6G using the enhancement effect of the substrate.